--- title: Home | Cellsonics description: Discover Cellsonics' innovative solutions for ultrasound therapy, advancing healthcare with cutting-edge technology and non-invasive treatments. --- [Skip to main content](https://cellsonics.com#main) [![Cellsonics](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/cellsonics-logo.svg) ![Cellsonics-logo](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Cellsonics-logo.svg)](https://cellsonics.com/home-new?hs_preview=ggrFiNrO-179903288415&hsLang=en) - [Resources](https://cellsonics.com/resources) - [About Us](https://cellsonics.com/about-us) - [Careers](https://cellsonics.com/careers) - [Blog](https://blog.cellsonics.com/blog) - [Contact Us](https://cellsonics.com/contact-us) Open main navigation Close main navigation - [Resources](https://cellsonics.com/resources) - [About Us](https://cellsonics.com/about-us) - [Careers](https://cellsonics.com/careers) - [Blog](https://blog.cellsonics.com/blog) - [Contact Us](https://cellsonics.com/contact-us) ![Play](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Play.svg) ![Pause](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Pause.svg) # Single Cell Sample Prep Re-engineered Ride the acoustic wave to enzyme-free tissue dissociation with SimpleFlow TM ![Simpleflow utilizes bulk lateral ultrasound (BLU)](https://cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Home/Simpleflow%20utilizes%20bulk%20lateral%20ultrasound%20(BLU).png?width=614&height=710&name=Simpleflow%20utilizes%20bulk%20lateral%20ultrasound%20(BLU).png) In contrast to enzymes that digest biological material to release cells, **SimpleFlow** utilizes acoustic waves that dissociate more cells without bias. **SimpleFlow** avoids the enzymes and heat typically used in traditional tissue dissociation methods, which are known to negatively impact the underlying biology and can selectively eliminate more sensitive cell types. As a result, **SimpleFlow** sources cell populations that are more representative of the native tissue to ensure higher biological relevance. ![Simpleflow utilizes bulk lateral ultrasound (BLU)](https://cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Home/Simpleflow%20utilizes%20bulk%20lateral%20ultrasound%20(BLU).png?width=614&height=710&name=Simpleflow%20utilizes%20bulk%20lateral%20ultrasound%20(BLU).png) In contrast to enzymes that digest biological material to release cells, **SimpleFlow** utilizes acoustic waves that dissociate more cells without bias. ![Simpleflow utilizes bulk lateral ultrasound (BLU) - Mobile](https://cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Home/Simpleflow%20utilizes%20bulk%20lateral%20ultrasound%20(BLU)%20-%20Mobile.png?width=345&height=345&name=Simpleflow%20utilizes%20bulk%20lateral%20ultrasound%20(BLU)%20-%20Mobile.png) **SimpleFlow** avoids the enzymes and heat typically used in traditional tissue dissociation methods, which are known to negatively impact the underlying biology and can selectively eliminate more sensitive cell types. As a result, **SimpleFlow** sources cell populations that are more representative of the native tissue to ensure higher biological relevance. ![HomePage-Female at Hood](https://cellsonics.com/hs-fs/hubfs/HomePage-Female%20at%20Hood.jpg?width=488&height=325&name=HomePage-Female%20at%20Hood.jpg) ## With SimpleFlow ![HomePage-Female at Hood](https://cellsonics.com/hs-fs/hubfs/HomePage-Female%20at%20Hood.jpg?width=488&height=325&name=HomePage-Female%20at%20Hood.jpg) **Dissociate tissue** without the use of enzymes Obtain **high-yield and high-viability** single-cell suspensions from a broad range of tissue types Retain **native cell populations** present in tissue prior to dissociation Process a sample **in minutes** and simplify cleanup procedures [Learn More About Cellsonics](https://cellsonics.com/learn?hsLang=en) Your browser does not support HTML video. ![Play](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Play.svg) ![Pause](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Pause.svg) ## Eliminate the tedious mixing of enzymes. Your browser does not support HTML video. ![Play](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Play.svg) ![Pause](https://cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Pause.svg) Simply add tissue, mince, and tilt to transfer, SimpleFlow's acoustics do the rest of the work. ## Maintain heterogeneity and leave no cells behind mouse mouse mouse mouse mouse mouse brain heart tumor lung spleen organoids ![scRNA-seq-all-1](https://cellsonics.com/hs-fs/hubfs/scRNA-seq-all-1.png?width=2000&name=scRNA-seq-all-1.png) ![scRNA-seq](https://cellsonics.com/hs-fs/hubfs/scRNA-seq.jpg?width=1386&height=1275&name=scRNA-seq.jpg) Read details Figure 1. A single cell RNA-seq comparison of whole mouse brain dissociated with SimpleFlow and a commercially available enzyme kit.   Tissue was dissociated on the SimpleFlow using the mouse brain protocol which ran for 4 minutes. The enzyme dissociation was performed according to the manufacturer's protocol.  Mouse brain dissociated with SimpleFlow demonstrated higher preservation of astrocytes and neurons. The enzymatic dissociated brain over-represents microglia, immune cells in the CNS that patrol the brain for damage and pathogens. This is indicative of stress and damage due to the dissociation process. These findings are consistent with published scientific literature! ## Yield more single cells with SimpleFlow ![live cell yield](https://cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Home/live-cell-yield.png?width=493&height=538&name=live-cell-yield.png) ![viability](https://cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Home/viability.png?width=493&height=538&name=viability.png) Read details Left. A comparison of 100 mg of adult mouse brain dissociated by either SimpleFlow or a commercially available enzyme kit.  The count of live cells per mg of tissue was determined by volumetric flow cytometry. Live cells were identified as Propidium Iodide (PI) negative excluding debris.    Right. A comparison of 100 mg of adult mouse brain dissociated by either SimpleFlow or a commercially available enzyme kit.  The percent viability was determined by flow cytometry and calculated as Propidium Iodide (PI) negative cells divided by total cells excluding debris. \*Assessed with PI by Flow Cytometry ![SimpleFlow-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/SimpleFlow-scRNA-Coming%20Soon.png?width=540&height=540&name=SimpleFlow-scRNA-Coming%20Soon.png) ![Enzyme-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/Enzyme-scRNA-Coming%20Soon.png?width=540&height=540&name=Enzyme-scRNA-Coming%20Soon.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. ## Yield more single cells with SimpleFlow ![Coming Soon-Live-Cell-Yield-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon-Live-Cell-Yield-1.png?width=540&height=540&name=Coming%20Soon-Live-Cell-Yield-1.png) ![Coming Soon540x540-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon540x540-1.png?width=540&height=540&name=Coming%20Soon540x540-1.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. \*Assessed with PI by Flow Cytometry ![SimpleFlow-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/SimpleFlow-scRNA-Coming%20Soon.png?width=540&height=540&name=SimpleFlow-scRNA-Coming%20Soon.png) ![Enzyme-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/Enzyme-scRNA-Coming%20Soon.png?width=540&height=540&name=Enzyme-scRNA-Coming%20Soon.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. ## Yield more single cells with SimpleFlow ![Front Page All Live cells](https://cellsonics.com/hs-fs/hubfs/Front%20Page%20All%20Live%20cells.png?height=2000&name=Front%20Page%20All%20Live%20cells.png) ![Front Page Tumor Viability](https://cellsonics.com/hs-fs/hubfs/Front%20Page%20Tumor%20Viability.png?height=2000&name=Front%20Page%20Tumor%20Viability.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. \*Assessed with PI by Flow Cytometry ![SimpleFlow-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/SimpleFlow-scRNA-Coming%20Soon.png?width=540&height=540&name=SimpleFlow-scRNA-Coming%20Soon.png) ![Enzyme-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/Enzyme-scRNA-Coming%20Soon.png?width=540&height=540&name=Enzyme-scRNA-Coming%20Soon.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. ## Yield more single cells with SimpleFlow ![Coming Soon-Live-Cell-Yield-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon-Live-Cell-Yield-1.png?width=540&height=540&name=Coming%20Soon-Live-Cell-Yield-1.png) ![Coming Soon540x540-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon540x540-1.png?width=540&height=540&name=Coming%20Soon540x540-1.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. \*Assessed with PI by Flow Cytometry ![SimpleFlow-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/SimpleFlow-scRNA-Coming%20Soon.png?width=540&height=540&name=SimpleFlow-scRNA-Coming%20Soon.png) ![Enzyme-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/Enzyme-scRNA-Coming%20Soon.png?width=540&height=540&name=Enzyme-scRNA-Coming%20Soon.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. ## Yield more single cells with SimpleFlow ![Coming Soon-Live-Cell-Yield-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon-Live-Cell-Yield-1.png?width=540&height=540&name=Coming%20Soon-Live-Cell-Yield-1.png) ![Coming Soon540x540-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon540x540-1.png?width=540&height=540&name=Coming%20Soon540x540-1.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. \*Assessed with PI by Flow Cytometry ![SimpleFlow-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/SimpleFlow-scRNA-Coming%20Soon.png?width=540&height=540&name=SimpleFlow-scRNA-Coming%20Soon.png) ![Enzyme-scRNA-Coming Soon](https://cellsonics.com/hs-fs/hubfs/Enzyme-scRNA-Coming%20Soon.png?width=540&height=540&name=Enzyme-scRNA-Coming%20Soon.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. ## Yield more single cells with SimpleFlow ![Coming Soon-Live-Cell-Yield-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon-Live-Cell-Yield-1.png?width=540&height=540&name=Coming%20Soon-Live-Cell-Yield-1.png) ![Coming Soon540x540-1](https://cellsonics.com/hs-fs/hubfs/Coming%20Soon540x540-1.png?width=540&height=540&name=Coming%20Soon540x540-1.png) Read details Our Application Scientists are currently developing this protocol. Please check back soon for the results. \*Assessed with PI by Flow Cytometry ## Improve downstream single-cell analysis with well treated cells ##### Tissue dissociation, a crucial step in preparing single cell suspensions for Flow Cytometry or Single-cell RNA-seq (scRNA-seq), plays a pivotal role in single cell analysis. ##### Conventionally, enzymatic incubations at 37°C have been the go-to method for tissue dissociation, despite numerous scientific publications highlighting the significant adverse effects of heat and enzymes on the cellular biology of individual cells. ##### Enzymatic dissociation has been repeatedly demonstrated to introduce artificial alterations in gene expression patterns, negatively impacting cell surface markers and impeding the accurate selection of cell subtypes via flow cytometry and single cell RNA-seq. ## Improve downstream single-cell analysis with well treated cells ##### Tissue dissociation, a crucial step in preparing single cell suspensions for Flow Cytometry or Single-cell RNA-seq (scRNA-seq), plays a pivotal role in single cell analysis. ##### Conventionally, enzymatic incubations at 37°C have been the go-to method for tissue dissociation, despite numerous scientific publications highlighting the significant adverse effects of heat and enzymes on the cellular biology of individual cells. ##### Enzymatic dissociation has been repeatedly demonstrated to introduce artificial alterations in gene expression patterns, negatively impacting cell surface markers and impeding the accurate selection of cell subtypes via flow cytometry and single cell RNA-seq. ## Latest Blog Posts ### [![Enzyme free tissue dissociation blog](https://cellsonics.com/hubfs/Inflammation-Resize.jpg) September 3, 2025 Preserving the True Immune Landscape: Eliminating Enzymatic Artifacts in Inflammation Research](https://blog.cellsonics.com/blog/preserving-immune-markers?hsLang=en) ### [![featured-image](https://cellsonics.com/hubfs/Spleen%20Blog.jpg) March 10, 2025 Immune Intergrity: Retain biological fidelity in single-cell spleen prep](https://blog.cellsonics.com/blog/protecting-immune-cell-integrity-keep-the-markers-enzymes-damage?hsLang=en) ### [![featured-image](https://cellsonics.com/hubfs/Enhanced-Mouse.jpg) February 11, 2025 Preserving the signal: Acoustic dissociation unlocks single-cell insights in CT26 tumor](https://blog.cellsonics.com/blog/preserving-the-signal-acoustic-dissociation-unlocks-single-cell-insights-in-ct26-tumor?hsLang=en) #### Sign up to be sent application notes. 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