Single Cell Sample Prep Re-engineered

Ride the acoustic wave to enzyme-free tissue dissociation with SimpleFlowTM

In contrast to enzymes that digest biological material to release cells, SimpleFlow utilizes Bulk Lateral Ultrasound (BLU) to create non-destructive, acoustic waves that dissociate more cells without bias.
 
SimpleFlow avoids the enzymes and heat typically used in traditional tissue dissociation methods, which are known to negatively impact the underlying biology and can selectively eliminate more sensitive cell types. As a result, SimpleFlow sources cell populations that are more representative of the native tissue to ensure higher biological relevance.

With the SimpleFlow system

  • Dissociate tissue without the use of enzymes
  • Obtain high-yield and high-viability single-cell suspensions from a broad range of tissue types
  • Retain native cell populations present in tissue prior to dissociation
  • Process a sample in minutes and simplify cleanup procedures

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Eliminate the tedious mixing of enzymes-simply add tissue, mince, and tilt to transfer

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SimpleFlow's BLU does the rest of the work

SimpleFlow BLU enzyme-free tissue dissociation workflow.

 

Improve downstream single-cell analysis with well treated cells

Tissue dissociation, a crucial step in preparing single cell suspensions for Flow Cytometry or Single-cell RNA-seq (scRNA-seq), plays a pivotal role in single cell analysis. Conventionally, enzymatic incubations at 37°C have been the go-to method for tissue dissociation, despite numerous scientific publications highlighting the significant adverse effects of heat and enzymes on the cellular biology of individual cells. Enzymatic dissociation has been repeatedly demonstrated to introduce artificial alterations in gene expression patterns, negatively impacting cell surface markers and impeding the accurate selection of cell subtypes via flow cytometry and single cell RNA-seq.

Maintain heterogeneity and leave no cells behind

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Figure 1. A single cell RNA-seq comparison of whole mouse brain dissociated with SimpleFlow and a commercially available enzyme kit.  

Tissue was dissociated on the SimpleFlow using the mouse brain protocol which ran for 4 minutes. The enzyme dissociation was performed according to the manufacturer's protocol. 

Mouse brain dissociated with SimpleFlow demonstrated higher preservation of astrocytes and neurons. The enzymatic dissociated brain overrepresents microglia, immune cells in the CNS that patrol the brain for damage and pathogens. This is indicative of stress and damage due to the dissociation process.

These findings are consistent with published scientific literature1

Yield more single cells with SimpleFlow

Flow Cytometry Yield

Figure 2a. A comparison of 100 mg of adult mouse brain dissociated by either SimpleFlow or a commercially available enzyme kit.  The count of live cells per mg of tissue was determined by volumetric flow cytometry. Live cells were identified as Propidium Iodide (PI) negative excluding debris. 06/19/2024

Flow Cytometry Viability

Figure 2b. A comparison of 100 mg of adult mouse brain dissociated by either SimpleFlow or a commercially available enzyme kit.  The percent viability was determined by flow cytometry and calculated as Propidium Iodide (PI) negative cells divided by total cells excluding debris. 06/19/2024