Non-enzymatic tissue dissociation in minutes
Cellsonics has re-engineered single cell sample preparation to capture the full potential of each and every tissue specimen. The innovative SimpleFlow™ tissue dissociation system enables researchers to capture important single cells in a user- and cell-friendly method.
With the SimpleFlow system
- Dissociate samples without the use of enzymes at a constant 5oC.
- Maintain the native gene expression profile across tissue and cell types.
- Recover the full spectrum of heterogeneous cell populations from solid tissue samples in 4-12 minutes of hands-free processing.
Enzyme free tissue dissociation as easy as...
Collect sample and load into SimpleFlow cartridge
Collect your sample and add it to the sample chamber. Do a quick mince with integrated mincer.
Run SimpleFlow protocol
Insert the cartridge and select a tissue specific preloaded protocol from the touchscreen, press start and walkaway.
Rapidly move on to Downstream Analysis
2 minutes later, remove your single cell suspension and move on to your downstream analysis.
Your tissue deserves the best, BLU™ energy dissociation is friendlier on your cells
Single-cell RNA-seq (scRNA-seq) is a powerful technique in obtaining transcriptome information from an individual cell. A critical upstream step to scRNA-seq is the dissociation of organs or tissues into a single-cell suspension. A popular method of tissue dissociation is the use of enzymatic incubations at 37°C, although this method has been shown to create artifactual changes in gene expression patterns.
Cell surface markers are critical for the accurate definition of cell subtypes and cell functions. These markers are required for accurate fluorescent labelling to enable analysis by flow cytometry. The enzymes used in tissue dissociation are known to negatively impact cell surface markers and inhibit cell subtype selection via flow cytometry.
The BLU energy technology used in SimpleFlow is superior to enzymes and heat. BLU is an acoustic energy based method that gently pulses minced tissue and separates that tissue into a single cell suspension. The dissociation process is short often taking only 2 minutes and the entire process is done at approximately 6oC. Data shows that using BLU provides a friendlier environment for the single cells that will be used in downstream application like scRNA-seq or cell selection via flow cytometry.
SimpleFlow’s enzyme free dissociation reduces induction of inflammatory and stress genes when compared to enzymatic methods
Improve cell selection and cell population recovery with SimpleFlow
FACS analysis of dissociated mouse liver cells with the SimpleFlow tissue dissociation system vs. enzymatic dissociation methods. Cell recovery of specific cell-types including CD45+ leukocytes (green boxes), and hepatocyte progenitor cells (blue boxes) was observed to be higher with the SimpleFlow system than with enzymatically-dissociated cells