Enzyme Free Tissue Dissociation to Single Cells in Minutes
Cellsonics has re-engineered single cell sample preparation to capture the full potential of each and every tissue specimen. The innovative SimpleFlow™ tissue dissociation system enables researchers to capture important single cells in a user- and cell-friendly method.
With the SimpleFlow system
- Cold dissociate tissue at a constant 6oC without the use of enzymes
- Retain the native gene expression critical for downstream applications like scRNA-seq and Flow Cytometry
- Obtain high yield and high viability single cell suspensions from a broad range of tissue types
- Batch process multiple samples with only 2 minutes of system time
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Enzyme free tissue dissociation as easy as...
Your cells deserve better, BLU™ energy dissociation is gentler on them
Tissue dissociation, a crucial step in preparing single cell suspensions for Flow Cytometry or Single-cell RNA-seq (scRNA-seq), plays a pivotal role in single cell analysis. Conventionally, enzymatic incubations at 37°C have been the go-to method for tissue dissociation, despite numerous scientific publications highlighting the significant adverse effects of heat and enzymes on the cellular biology of individual cells. Enzymatic dissociation has been repeatedly demonstrated to introduce artificial alterations in gene expression patterns, negatively impacting cell surface markers and impeding the accurate selection of cell subtypes via flow cytometry.
As an alternative to prolonged enzyme-based incubations at 37°C, the Cellsonics SimpleFlow introduces a rapid, enzyme-free tissue dissociation approach. SimpleFlow harnesses the power of Bulk Lateral Ultrasonic™ (BLU) energy, an acoustic energy-based technique that delicately pulses minced tissue to efficiently yield a single cell suspension. This dissociation process is brief, often completing within 2 minutes, all while maintaining the sample temperature at approximately 6°C. Data demonstrates that employing BLU technology creates a more favorable environment for the single cells destined for downstream applications such as scRNA-seq or precise cell selection via flow cytometry.
Explore our latest blog posts showcasing how SimpleFlow preserves crucial cell surface markers.
SimpleFlow’s enzyme free dissociation reduces induction of inflammatory and stress genes when compared to enzymatic methods
Improve cell selection and cell population recovery with SimpleFlow
FACS analysis of dissociated mouse liver cells with the SimpleFlow tissue dissociation system vs. enzymatic dissociation methods. Cell recovery of specific cell-types including CD45+ leukocytes (green boxes), and hepatocyte progenitor cells (blue boxes) was observed to be higher with the SimpleFlow system than with enzymatically-dissociated cells